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hi everyone, i have been facing the same problem as IPI. I can't transform the recombinant pFastBac HT-C into DH10BAC cells while my positive transformation control, pUC19 can be transformed into the DH10Bac cells. My recombinant plasmid (insert+pFAstBac) is 5996 bp in length, while pUC19 is ~2.6kbp, is it possible that the recombinant plasmid is too large to enter the DH10BAc cells? If this is the case, what would u all suggest?
And IPI, did you solved your problem? What factor did you find that could improve this problem?
Thank you everyone!
