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  2. → Viewing Profile: Topics: Damour

Damour

Member Since 10 Oct 2012
Offline Last Active Oct 19 2012 12:18 AM
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Topics I've Started

Expired tubing for cell culture?

15 October 2012 - 07:12 AM

What would you say to use expired tubing (silicone or c-flex) for cell culture in bioreactor? Has anyone ever noticed any effects from this?

thx

Declining productivity in long-term CHO cultivation, why!?

10 October 2012 - 06:37 AM

I apologize if this I am writing under wrong topic, but this is concerning mammalian cell culture in small scale STR's.

In long-term continuous cultivations (or shorter fed-batch cultivations with aged cells) I have observed a significant decrease in productivity when using mammalian cells.

I am referring to cells which have been continuously cultivated in a bioreactor for at least 1 month, or cells that have been passaged for at least 2 months and then inoculated into a bioreactor.

I have also observed a significant increase in cell growth rate.

How does cell age affect productivity and cell growth rate?

thank you

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