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Hi all,
I have transformed some dh10bac cells with pfastbac vector and plating them on x-gal, bluo-gal, kan, tet, and gentamycin plates. all i am getting is white colonies (which is good), but i was wondering if transposition can be so good that i dont get any untransposed colonies? i tried plating Dh10bac straight on to the plates above (minus gentamycin) and am still getting white colonies. is it possible that either IPTG or bluo gal is not working?
any help would be really appreciated
Cheers,
Imran
