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besides i dont think that there should be any difference in the results when you use different mediums yes your transfection efficiencies might differ, but once you have had successful transfection i dont think the results would change. if the current experiment is part of a larger study where you have mostly used EMEM, then I would definitely suggest to change it back to EMEM. It could save you a bit of embarrassment if somebody asks why you changed the media ? though it shouldnt really matter, but scientists are skeptical by nature.
The only way i can think of switching back to MEM is to probably mix DMEM and MEM and gradually increase the ratio to 100% MEM. If you have old stocks you could try to bring that up in MEM.
Good luck
