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vennela

Member Since 07 Oct 2012
Offline Last Active Oct 12 2012 09:25 PM

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cell culture, cytotoxicity

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drwho
07 Oct 2012 - 10:02

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dilution of cell suspension

07 October 2012 - 05:52 AM

hi
I want to seed a 96 well plate from a T25 flask having a volume of 4mL. Each well should have 10,000 cells in 100uL. I trypsinised the cells and added 1mL of medium to get a cell suspension. Its concentration is 1e6 cells/mL. So I took the entire 1mL of cell suspension and diluted it with 9mL of medium. From this I added 100uL into each well so that it contains 10,000 cells in 100 uL. Is this correct?