Is there any way to preserve spleen cells frm mice for few days longer to be analysed in flowcytometry later. Say 2 days maybe. Does it affect the viability of cells? . And whats the suggested amount of cell numbers needed for cell antibody staining work using flowcyto. ?
I use complete RPMI 1690 (with FBS and streptomycin ) as medium.
mjonMember Since 06 Oct 2012
Offline Last Active Oct 11 2012 02:01 AM
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My research interests
immunology, cell staining, proliferation, mushrooms extraction, flow cytometry, asthma,