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MariaDomenica

Member Since 04 Oct 2012
Offline Last Active Jan 25 2013 02:50 AM

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Immunology, biocompatibility

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Pangea
13 Jan 2013 - 07:01

Topics I've Started

macrophages and cytokines:

24 January 2013 - 11:47 PM

what method you use for your investigation? I m looking for a an alternative method to a classic use of elisa....

Suggestion for a book!

13 January 2013 - 05:42 AM

Hello to everybody
have you some soggestion about a manual or a nice book about cytokines or that is focus just on inflammation??

Thanks!!!

U937 and differentation to macrophages

12 October 2012 - 12:36 AM

Hi!
I´m starting to work with this cell and so to induce them to macrophages...my question is really sample:
You differentiate them in a flask or direct to the well plate?
What is for you the best desity of the cell to use?
I used 5X10^5 cells/ml with different concentration of PMA...5nM;10nM;15nM;20nM...but at 25 and 20nM they died,,,,