Question. My PAGE transfer (overnight) mysteriously stopped for a few hours (may be 2) after it ran for about 1h. It's a low percentage native gel (3.5%). Do you folks think the transfer quality will suck and my western blot will be all diffuse? The transfer buffer is pretty standard.
I'm beginning to think that someone has it out for me...
Tim ElliottMember Since 03 Oct 2012
Offline Last Active Oct 03 2012 05:27 PM
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