Google Sign in options
Remember me
This is not recommended for shared computers

Sign in anonymously
Don't add me to the active users list

Privacy Policy
Sign In
Create Account

Javascript Disabled Detected

You currently have javascript disabled. Several functions may not work. Please re-enable javascript to access full functionality.

yulia24

Member Since 03 Oct 2012
Offline Last Active Mar 14 2013 11:50 AM

Find content

Community Stats

Group Active Members
Active Posts 6
Profile Views 275
Member Title member
Age 40 years old
Birthday June 19, 1972
Gender
Female
Location
Canada

About me

My research interests
Molecular biology, cell biology

Contact Information

0 Neutral

Friends

yulia24 hasn't added any friends yet.

Latest Visitors

drwho
03 Oct 2012 - 11:49

Topics I've Started

Ponceau red problem: membrane turned red

14 March 2013 - 11:34 AM

Hi everybody

I've done Ponceau staining of my PVDF membrane after the transfer and got a lot of background binding that doesn't go away after washing with water. My whole membrane is red, with transferred bands visible on that red background. Does it mean there is no point continuing with this membrane? Anyone can explain why on earth did it happen?

Thanks

Y.

Low-temperature SDS-PAGE: tips and suggestions

08 January 2013 - 11:15 AM

Hi everybody

I'm attempting to check dimer-monomer ratio of eNOS protein using LT-PAGE approach. I've tried it twice and got very confusing and inconsistent results (some control samples showed two bands, while others did not). Here is how I did it. I'll appreciate any tips and suggestions.
I ran unboiled samples diluted in SDS loading buffer w/o DTT or b-mercapto. Both electrophoresis (I used pre-cast 4-12% NuPAGE gels) and the transfer were done in the ice bath, with temps always below 10C. Is there anything else I should be doing?

Thanks in advance!

Yulia

Best ECL detection reagents

03 October 2012 - 05:06 AM

Hi everybody!

What type of ECL detection reagents are you using and why? There are so many of them today it's difficult to choose. I'm looking for high sensitivity and long-lasting signal.

Thanks in advance!

yulia