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Hello to everybody!
I have some trouble with the western blot!
I just got bands of high molecular weight protein and nothing about low molecular weight..
But i was looking for histones modifications,
I did the whole protein precipitation and not the acid one, can it be because of it, so i lost all the histones? Or it doesnt matter that much??
Any suggestion on tranfer tecnique tips?!
Thank you all!
Stefania.
