Hello!
I have a question, i had some problems similar to you! So the extraction o whole cell is not a problem when looking for histones? Because i did it, but i dont find the blots for the LW proteins i just find blots for the HW..and some of my labmates told me it was a mistake not doing the acid extraction insted of the normal one with lysis buffer!
Thank you
Hello!! Thanks for the reply! I first did the extraction with the lysis buffer ( with protease inhibitors), and i did the SDS page i loaded a very little ammount of protein, but it cames from the quantitation protocol. I runed at 10V overnight..maye i have lost my protein? Or the didnt transfer?!? Thank you so much!!
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