I hope you can help I have read many topics on this issue but thought I would ask for some help.
I am trying to isolate RNA from a small numbers of cells ~1000 (human samples). In my trial experiments using cell lines I managed to get usable quantities and quality RNA using Guanidinium thiocyanate-phenol-chloroform extraction (TRIZOL) with an additional ETOH sodium acetate precipitation, also tried a butanol concentration set with good results. I was able to repeat this in a number of differnt cells types.
However now I have tried it on the real samples I have good quantity and good 260/280 values however my 260//230 values are low ~1.3-1.5. I am at a bit of a loss about what to do. I will be unlikely to get more of these cells for a long time if at all.
Any advice on how I can improve my 260/230 values. I will want to use the RNA for standard real time PCR no arrays or anything like that.
Any suggestions more than welcome.
drwhoMember Since 27 Sep 2012
Offline Last Active Nov 20 2012 02:06 AM
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receptor pharmacology, protein protein interactions, drug development