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Hi all
I hope you can help I have read many topics on this issue but thought I would ask for some help.
I am trying to isolate RNA from a small numbers of cells ~1000 (human samples). In my trial experiments using cell lines I managed to get usable quantities and quality RNA using Guanidinium thiocyanate-phenol-chloroform extraction (TRIZOL) with an additional ETOH sodium acetate precipitation, also tried a butanol concentration set with good results. I was able to repeat this in a number of differnt cells types.
However now I have tried it on the real samples I have good quantity and good 260/280 values however my 260//230 values are low ~1.3-1.5. I am at a bit of a loss about what to do. I will be unlikely to get more of these cells for a long time if at all.
Any advice on how I can improve my 260/230 values. I will want to use the RNA for standard real time PCR no arrays or anything like that.
Any suggestions more than welcome.
