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R.Mufti

Member Since 26 Sep 2012
Offline Last Active Sep 28 2012 08:23 AM
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Topics I've Started

Calcium imaging

26 September 2012 - 05:35 PM

I need some clarification please
I use Fluo-4 Calcium fluorescence dye to measure calcium waves.
-Why it is not accurate to measure the amplitude? we only can measure frequency and % of cells firing waves.

question about protein expression normalization.

26 September 2012 - 11:38 AM

Hi everyone, I am a new member and I found this site is really helpful.
When I normalize (intact vessels) my samples,  should I choose actin or the total protein?
I was told that I should use total protein in the case if actin was affected by any treatment or condition, am I right?

Thanks

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