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# uzalive

Member Since 25 Sep 2012
Offline Last Active Oct 03 2012 11:48 AM

### dilution question

03 October 2012 - 07:42 AM

Example 1: Suppose you have 3 ml of a stock solution of 100 mg/ml ampicillin (= C1)  and you want to make 200 ul (= C2)of  solution having 25 mg/ ml (= V2).  You need to know what volume (V1) of the stock to use as part of the 200 ul total volume needed.

I don't understand why 3ml is given?  When I tried to do this problem I thought the V1 was 3ml.  I know the formula that I am to use to solve this problem which is v1c1=v2c2.

Can someone clarify this

### 100 micro molar to 250 micro molar

25 September 2012 - 11:31 AM

1. You are provided with an antibody solution Ab that has a concentration 600 ug/ul. For lab it is necessary to make thefollowing dilutions.

a.  10 ul of 600 ug/ul Ab + 190ul of buffer to make a 1:20 dilution at _________ ug/ul.

b.  20 ul of 1:20 +40 ul of buffer to make a 1:60 dilution at __________ ug/ul.

c.  5ul of 1:60 + 5ul of  buffer to make a ________ dilution at _________ug/ul.

It seems I am missing dome concepts.  Could someone help?