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# uzalive

Member Since 25 Sep 2012
Offline Last Active Oct 03 2012 11:48 AM

### In Topic: 100 micro molar to 250 micro molar

29 September 2012 - 10:02 AM

You have an antibody solution that has a concentration of 600 ug/ul.  You want to make th following dilutions.

10 ul of 600 ug/ul Ab +190 ul of buffer to make a 1:20 dilution at ________ ug/ul.

This is how I did it.

1.  600 ug/ul times 10 ul= 6000 ug

2.  6000 ug/200ul= 30ug/ul

I know that this answer is correct because I checked.  I don't understand how this gives you a 1:20 dilution.  Why is the 1:20 given?  I did not use it in my calculations.  It seems that I am missing some concept.  Can someone clarify this.  I would have thought that you would divide 30 ug/ul by 20.

Uzalive

### In Topic: 100 micro molar to 250 micro molar

25 September 2012 - 02:08 PM

I did not answer the question at first this is how I did it

a.  10ul times 600ug/ul =6000 ug

add the buffer, which is 190 ul, to 10ul to get 200 total ul.

6000ug/200= 30 ug/ul

I don't understand how do we know that it is 1:20 dilution.

b.  600ug/ul/ 20=30 ug/ul

30 ug/ul times 20ul=600ug/ 60 ul= 10 ug/ul

I don't understand why the it is a 1:60 dilution.  I don't understand why 1:60 dilution was given.  I did not use it in solving the question, but my answer is correct.

Am I missing something.

c.  600ug/ul/60=10ug/ul times 5ul=50 ug

50 ug/10ul= 5ug/ul   I could not figure out the dilution.