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Greetings,
I've been doing some silver staining recently and some of the bands in my gels have just stopped migrating. They cross the border between the stacking and resolving gel just fine but then seem to stop migrating. I've cast new gels and changed sample buffers. The molecular weight markers are migrating just fine, any suggestions as to what's causing this. This is a new phenomenon so any help resolving it would be a big help.
