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mdfenko, on 16 November 2012 - 11:01 AM, said:
if the standards ran well then it is most likely not something with the gel or buffer. it is most likely something with the sample. since all of the samples are running similarly then it is likely due to the buffer that the sample is in or the sample loading (laemmli) buffer. is the sample buffer old? the sds may be decomposing.
try making a fresh sample loading buffer (use a different lot of sds).
try making a fresh sample loading buffer (use a different lot of sds).
My problem was fixed by making fresh sample buffer. Thank you.
