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eppie123

Member Since 21 Sep 2012
Offline Last Active Sep 24 2012 08:45 AM

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Elizabeth Epps

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2xzwei
28 Sep 2012 - 00:06

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In Topic: Help with high background (have tried most trouble shooting)

21 September 2012 - 10:31 AM

Maybe try increasing your primary addition to 1:10,000 and/or decreasing your secondary to 1:5000. Maybe check your antibodies? Also, maybe try using nitrocellulose membranes instead of PVDF?