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I am trying to perform a Quikchange mutagenesis using PCR. I have been able to get this to work in the past but now I'm having some trouble.
I have been able to get a mutation to work using one plasmid, but I got another plamsid with the same sequence (only the protein is tagged with an epitope for purification) and the PCR mutagenesis does not work.
Any thoughts as to why the same mutagenesis works on the same protein sequence (I have confirmed it's the same sequence) in one plasmid but not work in a differen plasmid?
Thanks
