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ColdOven14

Member Since 18 Sep 2012
Offline Last Active Oct 02 2012 05:43 PM
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Topics I've Started

Non-fluorescent live/dead stains for prokaryotes

18 September 2012 - 07:54 PM

I will be researching in Antarctica at the end of the year and as such, I wont have an epifluroescence (only a compound) microscope available.
I need to conduct viability counts of prokaryotic bacteria, most likely using a haemocyctometer, and am looking for a stain which could be used for this purpose. I've looked into Trypan blue but have a few specific questions about it.
1) Can it be taken up by prokayotic cells?
2) Is there a counter stain available which I can use to for counting my live bacteria?
Any help in regards to trypan blue or alternative stains would be greatly appreciated.

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