I will be researching in Antarctica at the end of the year and as such, I wont have an epifluroescence (only a compound) microscope available.
I need to conduct viability counts of prokaryotic bacteria, most likely using a haemocyctometer, and am looking for a stain which could be used for this purpose. I've looked into Trypan blue but have a few specific questions about it.
1) Can it be taken up by prokayotic cells?
2) Is there a counter stain available which I can use to for counting my live bacteria?
Any help in regards to trypan blue or alternative stains would be greatly appreciated.
ColdOven14
Member Since 18 Sep 2012Offline Last Active Oct 02 2012 05:43 PM





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