Hello, I am desparately trying to reproduce colony formation assays in A2780 (ovarian carcinoma) cells, but eventhough I am following the protocol, the results vary like crazy. One big problem is, that I oftrn don't even get nice colonies - the colony numbers are much lower than they should be and the colonies are smaller as well. Also, I don't see the phenotype I expect but rather the opposite (comparing knock-down of a gene and control). Sometimes though I do see what I want to see - but just very rarely and I can not tell why because I am not aware of having changed anything. Please help if you can! Thanks, Angelika
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