greetings i am new here, and i'm sorry if this is not the correct place but if someone can help me lighting up my path, i'm just a student in biology but i'am helping one of my teachers (well,i'm very newbie, so may be he is helping me) , and i'm trying to figure out the use of some substances (my teacher knows, but he says "figure it out by yourself, then i will ask you") in 2 Lysis solutions from a protocol for comet assay that i got, the first lysing solution:
reactive: use for maybe?
*NaCl : osmotic shock i guess.
*EDTA: avoids the degradation of DNA while the preparation and in the electrophoresis run.
*DMSO: protects the cells
*triton X100: lyse the cells
at 4°c, may be (i think...again) is for inhibits the enzymes from the acrosome from the sperm cell (proteases. hyaluronidase...etc)
well i know that all the lysing solutions are made for lyse the cells, and normally the compunds in them are salts and detergents, but reading i think that is the use of them.
the second lysing solution
NaCl:the same osmotic shock?
Na2EDTA:is obvious a detergent but, i don't know...
tris:protects the DNA from the pH changes.
proteinase K:inhibits the DNAases, and other enzymes.
DTT:protects the cells from oxidation damage
at 37.7°c, i think is for the proteinase K.
well, if someone can help me, thanks in advance, and sorry for my bad english...
jesscallerosMember Since 12 Sep 2012
Offline Last Active Jan 01 2013 12:05 AM
- Group Members
- Active Posts 4
- Profile Views 335
- Member Title member
- Age Age Unknown
- Birthday Birthday Unknown
My research interests
Genetics, biology,chemistry,toxicology,virology And everything interesting (almost all)
jesscalleros hasn't added any friends yet.
13 Sep 2012 - 03:45