jesscalleros

greetings i am new here, and i'm sorry if this is not the correct place but if someone can help me lighting up my path, i'm just a student in biology but i'am helping one of my teachers (well,i'm very newbie, so may be he is helping me) , and i'm  trying to figure out the use of some substances (my teacher knows, but he says "figure it out by yourself, then i will ask you") in 2 Lysis solutions from a protocol for comet assay that i got, the first lysing solution:
reactive: use for maybe?
*NaCl : osmotic shock i guess.
*EDTA: avoids the degradation of  DNA while the preparation and in the electrophoresis run.
*DMSO: protects the cells
*triton X100: lyse the cells
at 4°c, may be (i think...again) is for inhibits the enzymes from the acrosome from the sperm cell (proteases. hyaluronidase...etc)

well i know that all the lysing solutions are made for lyse the cells, and normally the compunds in them are salts and detergents, but reading i think that is the use of them.

the second lysing solution
NaCl:the same osmotic shock?
Na2EDTA:is obvious a detergent but, i don't know...
tris:protects the DNA from the pH changes.
proteinase K:inhibits the DNAases, and other enzymes.
DTT:protects the cells from oxidation damage
at 37.7°c, i think is for the proteinase K.

well, if someone can help me, thanks in advance, and sorry for my bad english...