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#14876 Free Statistics
Posted
on 07 February 2009 - 07:02 PM
#13566 Handbook of Biological Statistics
Posted
on 28 January 2009 - 12:02 AM
#141416 Comet assay sperm cells
Posted
on 13 September 2012 - 04:50 PM
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Part 1- NaCl - for osmotic shock you would need a concentration that is above or below the normal physiological range - Normal saline is 150 mmol/l or 0.9%.
EDTA: chelates metal ions, which helps inhibit those enzymes that have a metal co-factor.
DMSO: This one is tricky - it's actually to scavenge free radicals The protectant function only comes into play at really low temperatures where it prevents ice crystal formation.
Triton X-100 is a detergent, used to pop the membranes.
Part 2:NaCl and EDTA are the same as above. EDTA is not a detergent
Tris: buffering is correct. Tris buffers strongly at pH7 or there abouts.
Proteinase K: OK.
DTT is there to denature proteins, and probably to help prevent secondary structure in the DNA.
37 is the optimal temperature for proteinase K.
I'm also shifting this to one of the homework forums...
Part 1- NaCl - for osmotic shock you would need a concentration that is above or below the normal physiological range - Normal saline is 150 mmol/l or 0.9%.
EDTA: chelates metal ions, which helps inhibit those enzymes that have a metal co-factor.
DMSO: This one is tricky - it's actually to scavenge free radicals The protectant function only comes into play at really low temperatures where it prevents ice crystal formation.
Triton X-100 is a detergent, used to pop the membranes.
Part 2:NaCl and EDTA are the same as above. EDTA is not a detergent
Tris: buffering is correct. Tris buffers strongly at pH7 or there abouts.
Proteinase K: OK.
DTT is there to denature proteins, and probably to help prevent secondary structure in the DNA.
37 is the optimal temperature for proteinase K.
I'm also shifting this to one of the homework forums...
