I just finished a western today where I loaded cell lysates (each lane had the same cell line but with different plasmids in them) and I blotted using the same primary and secondary for all lanes. After development, I saw non-specic bands that were present in some lanes but not in others. Any idea to what is happening?!
Elia El HabreMember Since 12 Sep 2012
Offline Last Active Sep 18 2012 01:48 AM
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13 Sep 2012 - 03:45