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soluble CD3 is good, in combination with DCs or splenocytes.
my experience with a CD4 T cell hybridoma (B5) is that it's very sensible and produce large amounts of IL2.
I stimulated the cells exactly as you did: DCs and peptide, the numbers where 50,000 DCs with 100,000 T cells....but 50,000 T worked as well....
