hi all, i am trying to work out how to determine the promoter sequence for rat CYP2E gene, and have followed all the steps in the post how to find the promoter, through to the blat search, however when i try to get the alignment page, it looks nothing like the example BRCA1, i dont understand why? can anyone help?! There are no lines to suggest alignment? any help would be appreciated!
Hi! i am really stuck and could do with some help- im trying to design methylation specific primers using methyprimer, and i input the sequence and it designs primers but also says no cpgs found- i dont get it? it is this ok or not? please forgive my ignorance i seem to be going round in circles!
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