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Hi and happy new year,
I am going to prepare pyruvte kinase enzyme for section of assay process of starch synthase enzymic activity .
Pyruvate kinase enzyme is as a crystalline suspension in 3.2 M ammonium sulfate, pH 6.0. (5000 unit ).
for each assay 1.2 unit of this enzyme must be used. I know how calculate the amount of µL that is need for each assey and i know how prepare the work stock, but i don't know how can i prepare an solution of purchased enzyme as purified stock. I want to know the preperation method of purified stock of enzyme that is as a crystalline suspension. (form of purchased enzyme is crystalline particles). Prompet reply will be really appreciated.
