I am not analyzing protein but using Postaglandin 2. Since I was getting this problem I called up the supplier and they told me that the solvent I dissolved the PGE2 in (acetonitrile) might have caused them to degrade because of change in pH.
But I m still confused as if the sample has degraded the degraded substance should have show a peak in the chromatogram which was not seen.
Zeenat TinwalaMember Since 09 Sep 2012
Offline Last Active Sep 11 2012 06:26 AM
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