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  2. → Viewing Profile: Posts: Surya r

Surya r

Member Since 07 Sep 2012
Offline Last Active Sep 21 2012 03:28 AM
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Posts I've Made

In Topic: protein extraction from human tissue (very small) to do ELISA

20 September 2012 - 03:53 AM

Thanks, but not really. The buffer in which we collect is very important. RIPA buffer is no good and  same with any buffer with strong detergents. I cannot snap freeze my sample. I am trying to find a good method  right from the collection stage to a final stage where I could run an ELISA. Mnay thanks

In Topic: protein extraction from human tissue (very small) to do ELISA

20 September 2012 - 01:14 AM

Thanks Curtis. I searched but couldn't find any method that can be used to do ELISA. Could you please help me

In Topic: Protein precipitation from RIPA buffer to perform ELISA

12 September 2012 - 06:57 AM

thank

View Postmdfenko, on 10 September 2012 - 08:09 AM, said:

check these web pages for a quick description:

fundamentals of dialysis

and

dialysis faq
thank you

In Topic: Protein precipitation from RIPA buffer to perform ELISA

10 September 2012 - 01:12 AM

Thank you. Could you please elaborate or send me a protocol to do it. Thanks in advance

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