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Not Telling
It's just that is seems to skip a lot of clarifying information.
Example:
Step 5. Which supernatants from step 4 is he refering to? I Guess both of the "wash" steps.
Step 6. Centrifuge again, but for how long, both 5 and 10 minutes centrifugation has been used in previous steps.
Step 7 The author wants me to "resuspend in the same buffer as above", only we used two different buffers above (I guess he means the one with glycerol).
I trying it out as soon as my patient fibroblast samples have grown to a suffient total mass. Hope it will work .-)
