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metionina

Member Since 05 Sep 2012
Offline Last Active Apr 14 2013 06:22 AM

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Group Active Members
Active Posts 23
Profile Views 921
Member Title member
Age Age Unknown
Birthday Birthday Unknown
Gender
Female
Location
Cambridge, United Kingdom

About me

My research interests
mycobacteriology, membrane protein, structural biology

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Latest Visitors

Akila Wijerathna Yapa
10 Nov 2012 - 11:37
drwho
28 Sep 2012 - 12:24
ascacioc
25 Sep 2012 - 13:54
Nayeem991
20 Sep 2012 - 22:08
Rchqing
17 Sep 2012 - 17:22

Topics I've Started

mass spectrometry and tryptic digestion on membrane proteins

24 September 2012 - 08:32 AM

I have a band on my Coomassie blue stained SDS-PAGE and I want to cut it in order to identify the protein by mass spectrometry after tryptic digestion. If my purification is correct, this is a membrane protein.
For the tryptic digestion, do you use the protocol for soluble proteins?

Hello everybody and thanks for joining the group!

14 September 2012 - 03:36 AM

Hello everybody and thanks for joining the group! Posted Image

Hope it can be helpful for all of us.

If you have any idea, suggestion or complain regarding our group, feel free to share your opinion!

A forum for discussing membrane proteins?

10 September 2012 - 12:49 AM

Interest in membrane proteins becomes higher every day and some 70% of current drug targets are membrane proteins. But working with this type of proteins remains very difficult, only few structures are available in the PDB and membrane protein researchers are often alone with their problems (solubility - aggregation - expression and purification - electrophoresis techniques - structure determination - etc).

I didn't find any forum or group specific for these proteins in the web and I'd like to propose to create a group here.
Is somebody interested in that idea?