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metionina

Member Since 05 Sep 2012
Offline Last Active Apr 14 2013 06:22 AM

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Active Posts 23
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Cambridge, United Kingdom

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My research interests
mycobacteriology, membrane protein, structural biology

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Akila Wijerathna Yapa
10 Nov 2012 - 11:37
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Posts I've Made

In Topic: mass spectrometry and tryptic digestion on membrane proteins

28 October 2012 - 06:27 AM

thank you KarenLL!

In Topic: Thrombin - how to use it in the best way?

28 October 2012 - 06:22 AM

I use 100 U of thrombin and it works well, with no problem for gel staining.
Your option 2 I think it's the better way to cut and then recover your protein.
There is also a thrombin (I forgot who sells it) that is bound to agarose beads: an easy way to isolate the thrombin after digestion.

In Topic: silent mutations

05 October 2012 - 07:27 AM

some examples found in pubmed
http://www.ncbi.nlm....pubmed/17001296
http://www.ncbi.nlm....pubmed/10775534
http://www.ncbi.nlm..../pubmed/9197418

and more general articles
http://www.nature.co...s061218-12.html
http://seedmagazine....und_of_silence/

In Topic: silent mutations

05 October 2012 - 04:01 AM

I think yes.
A silent mutation in a gene doesn't change the amino acid of the protein, but you have to consider also the regulation mechanisms associated with the gene (the mutation may cause for example a different splicing or a different regulation of the expression of the gene).
Maybe you can look for some examples in pubmed.

In Topic: mass spectrometry and tryptic digestion on membrane proteins

24 September 2012 - 01:05 PM

I read somewhere that hydrophobic peptides can bind the surface of your tubes so you have to use specific tubes if you don't want to lose your peptides and that it was better to use another acid instead of TCA. Then somebody told me about heating as technique to obtain better results during the vaporisation or the HPLC (in LS-MS-MS).
But I'm not sure of that and I never found a real protocol.

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