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Hi!
I have a problem about very basic cell lysis. I lysed human cells (a cultured cell line) with SDS-tris buffer. The solution was cloudy, so I thought it was due to a kind of SDS precipitattion (carry over of potassium etc from media/PBS etc?), so centrifuged the sample > 10,000 x g for 10 min etc. Unexpectedly, instead of finding some precipitation at the bottom of the tube, there was some white marerial floating over the solution. The white material appeared to be some lipid but I could not be sure and do not know what it was and why it appeared (I never have observed such floating material after hundreds of cell lysates I made before). Any suggestions, please? Thank you.
Schu
