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I will do my 1st test run only once...........but then if it works I will always do it at least in triplicate.........especially if enrichments are lower. I decided to scrap my GFP ChIP till next week..........I noticed that the ab290 is unpurified serum so they dont actually give you the concentration of specific serum. However, they do give you the overall concnetration.............and it's huge 5ug/ul.........so I think I'm going to only add 1ul of antibody in 1ml of diluted chromatin (~2x10^6cells)..........the reason im only going to add 1ml is that my protein A beads only bing 5-6ug of IgG per 20ul..........which is what I add to each IP............so long story short, I will hopefully have something good to report next week.
#142351 abcam 209 GFP antibody for CHIP- is there an alternative GFP antibody useful for
Posted
on 27 September 2012 - 08:20 AM
