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Mohamed 1984

Member Since 29 Aug 2012
Offline Last Active Dec 09 2012 12:00 PM

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Community Stats

Group Active Members
Active Posts 12
Profile Views 381
Member Title member
Age 28 years old
Birthday September 29, 1984
Gender
Male
Location
Germany
Hobbies
Virology , Avian influenza virus, Molecular biology

About me

My research interests
Virology - molecular biology - Avian influenz virus

Contact Information

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Hemavet
04 Dec 2012 - 03:30
absarbiotech
11 Oct 2012 - 21:22
Christo K
17 Sep 2012 - 05:43
vitalgene
16 Sep 2012 - 12:51
pcrman
15 Sep 2012 - 17:56

Topics I've Started

Real time PCR melting curve

03 December 2012 - 05:31 PM

Actually, i face a problem with real time PCR of miRNA. I tried the primer of one type of miRNA two time and i observed a one typical bell shape curve and other multi peak curve . Could any one tell me about the causes of multi peak melting curve in real time PCR

qPCR, Appearance of amplification curve for my minus RT control

15 September 2012 - 04:27 PM

Iam really get shocked yesterday. The reason behind is that i run an experiment of qPCR to check the quality of hosue keeping gene. The amplification curve of the sample was perfect but i found that my minus RT control showed a normal amplification while iam sure that i did not miss add any cDNA sample to it. What may be the cause ? and how i can correct?

Thanks in advance

How to quantify virus by PCR

29 August 2012 - 04:43 PM

i want to correlate the quantity of microRNA and influenza virus , so i isolated the total RNA from experimentally infected lung. now i convert all the isolated RNA to cDNA. iam preparing to measure both the amount of miRNA 223 and miRNA21 and one of influenza genes

So my question: How can i measure the quantity of influnza virus in my sample so that i can correlate it to the quantity of my miRNAs? please in details.