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Mohamed 1984

Member Since 29 Aug 2012
Offline Last Active Dec 09 2012 12:00 PM

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Group Active Members
Active Posts 12
Profile Views 410
Member Title member
Age 28 years old
Birthday September 29, 1984
Gender
Male
Location
Germany
Hobbies
Virology , Avian influenza virus, Molecular biology

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My research interests
Virology - molecular biology - Avian influenz virus

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#146240 Real time PCR melting curve

Posted casandra on 03 December 2012 - 08:52 PM

Mohamed 1984, on 03 December 2012 - 05:31 PM, said:

Actually, i face a problem with real time PCR of miRNA. I tried the primer of one type of miRNA two time and i observed a one typical bell shape curve and other multi peak curve . Could any one tell me about the causes of multi peak melting curve in real time PCR

multiple peaks mean multiple products which can be non-specific amplicons or usually from primer dimers but I'm not sure about miRNA primers- are they from a publication?...(posting an image will be more helpful) and what about your NTCs (no peaks?) You can also search for threads in this forum addressing this same or related issues....