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I have been doing ChiP assay using a kit from Upstate, millipore.
I completed the entire procedure and did PCR for GAPDH promoter sequence with positive control (RNA Pol II antibody) and negative control (Mouse IgG).
Surprisingly i got a band in both positive and negative
. The band was fainter in negative, but then there was a distinct band after PCR.Any help on what i should do right???
ivap
