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Hi all,
I want to resuspend colonies from a mixed culture (environmental, no strains) that I have on a plate (M9 agar) and respread it on a new plate to do a little comparison on how well they will grow on different solid media. Problem is I am not sure how much (both in volume and OD) would normally be ok for spreading on a plate in order to avoid a mess of growth or an empty plate. Is there a range of "suitable" ODs for plating?
Thanks in advance,
Omni
