Hi! I´m trying to set up a ChIP protocol for mice liver tissue, and I wanted to know how much DNA I need to use for each IP. Is 7ug too much or too little material per IP? And 50 ul of beads would be enough for this concentration of DNA? Do you think I can trust the concentration values I get in the Nanodrop after sonicating? Otherwise, how do you calculate the amount of DNA you add per IP? You just calculate it according to the mg of tissue needed per IP? Thanks so much, I am quite lost, so I would be glad if you could give me any suggestions.
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