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micronagu

Member Since 21 Aug 2012
Offline Last Active Mar 14 2013 06:50 PM

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Infection biology, Microbiology, cancer biology

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laurimoilanen
30 Aug 2012 - 23:35

Topics I've Started

Western blotting

22 January 2013 - 05:49 AM

Hi every one,

I am doing my research on Helicobacter pylori. After lyse the infected mammalian cell, I am doing western blotting to check the injected virulent protein. for that I am using rabbit polyclonal abs, and am experiencing non specific binding and lot of noise background, mean while i didnt experience any other non specific binding while using monoclonal abs for other targets.

Am doing 1:30 hrs membrane blocking, abs incubation for over night, 1:30 hrs abs blocking and 1:30 hrs secondary ab incubation with 3x10 min washing. some times i didnt got any noise but many times i am getting noise background and non specific binding.

How to overcome this problem.

Hope for looking valuable suggestions.

thanks

micronagu

can I dilute the drug with DMEM

21 August 2012 - 02:28 AM

hi there,
wishes, I am using a drug that should be dissolve in DMSO. I am working for to detect the cytotoxicity of this drug on mammalian cells by MTT assay. I have reffered from the research articles and researchers, that for doing this experiment DMSO concentration should be same or below of 0.1 %. So, may i dilute the drug by using DMEM from stock solution which is made up of DMSO.
Looking for your kind reply.
thank you,
micronagu