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Shannah

Member Since 20 Aug 2012
Offline Last Active Jan 14 2013 12:49 AM

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Tissue Homogenate

14 January 2013 - 12:49 AM

I'll have to make a homogenate of tissue (aorta) and now I face the problem what the best method to store them.
I cannot not use them immediatly so I have to collect them. Is it better to store the aorta as whole or as homogenates? I'm a little bit afraid of protein degredation because I have to measure different protein levels of the homogenates

Thanks for your help.

oxLDL-IC Assay

20 August 2012 - 01:06 AM

Hi everybody,

I'm looking for a method or protocol to obtain oxidized LDL Immune complexes. I Already hava a protocol to get oxidized LDL using copper but non to make Immune complexes with IgG or IgM out of it. I want to measure the produced oxLDL-IC and therefore need some kind of binding Assay.

Does someone know how to make it ? Thanks a lot

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Shannah

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