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I am doing a project with human serum, as i need to remove albumin., i tried TCA preciptation, according to a new protocol saying that TCA preciptation can reduce albumin content.. i used 4 volumes of TCA/acteone. after the entrie procedure i got a pellet. the problem is i tried to dissolve the pellet in 1M tris pH 6.8 but I cudnot dissiolve. After TCA perciptation i need to go for protein estimation by lowrys method then SDS-PAGE then 2D gel then mass spectrometry.. I have read tht we can dissolve pellet in 2X SDS-Loading buffer... But as i need to go for protein estimation by lowry's i don't think so i can go for SDS loading buffer... Pls do just me a method were i can continue through my entire project....
Anu
