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  2. → Viewing Profile: Posts: MScMini

MScMini

Member Since 14 Aug 2012
Offline Last Active Jan 08 2013 02:40 AM
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Posts I've Made

In Topic: Can I make an SDS stock in media and store?

07 January 2013 - 03:50 AM

Thanks for the advice!

In Topic: Avoid SDS in thermal lysis: whole blood?

03 January 2013 - 11:15 PM

Pending on what you want to lysate, but for for PBMC separation I start with PBS (without Mg and Ca) with 2mM EDTA.
Hope you find it helpful!

In Topic: WANTED: Suggestions on how to decontaminate with formalin/formaldehyde

17 September 2012 - 03:43 AM

After a week of waiting and wishing; partial success..
I had no growth in the dishes I put in there for test, but in the water pan..
I am refilling with autoclaved water as suggested by Akdor but I am afraid the
damage is already done..
Could the problem be the water system??

In Topic: WANTED: Suggestions on how to decontaminate with formalin/formaldehyde

06 September 2012 - 11:31 PM

I have no possibility to autoclave panels from the incubator.. I use only Millipore ultrapure water from an Elix3 which is proprely maintained.
There is no automatic decontamination program in the incubator (nuaire 5500).
As i said, there is a firm coming to ozone treat the room today (and the whole weekend) so I hope it will be clean on monday ;)

Fungi grows in layers (or so a green biologist tells me) and may need several cleanings before being completely removed.

Anyway I put my new hope to the ozone-guy..

In Topic: WANTED: Suggestions on how to decontaminate with formalin/formaldehyde

06 September 2012 - 06:04 AM

The incubator is sitting empty, part from a dish and a flask which contained only media when I put them there.
Now they contain more.. *sigh*

Water is replaced each friday (by me).
I set up a routine to clean the incubator once a month, but I've only been here 3 months..
I now suspect that my boss is less than happy, but I ordered a company to come and ozone spray the intire incubator room including the incubator.
It was cheap but I hope that it will end atleast some of my problems.

Once the ozone has cleared I will set up another dish and flask to check for spores.

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