Find content
Not Telling
Posts I've Made
In Topic: Need help for prepping western samples with NP-40
09 August 2012 - 09:57 PM
Curtis, on 09 August 2012 - 09:30 PM, said:
This is a common problem and has been asked many times on this forum before. You need to add 1-2 ul DNase to your sample. Even sonication won't help you. But if you do all your work on ice you are less likely to see that gooey clump.
Cool, and the np40 doesn't hinder the DNAse? Also, to make myself clear I'm collectin supernatant and not the nuclear pellet. Thanks!
