ascacioc

If Person is more into lonely life and more into science or special activity he has probably less chance of understanding and conversing topic of general interest. For starting any conversation backup data is most important source, even to come things from subconscious mind this data is needed. Such person should be more into social life then. Giving time to extra curriculum activity, reading some non-academic interest stuff should help.

Please note I'm not judging.

One advise:

make sure you have anough of "proof".
If you cant catch her in the act, make sure you have "proof" that shows something is going wrong.
Use this to in a discussion with your boss.

And one hint: you dont need to accuse her right away, just collect the "proof", ask your boss to have a chat with you to discuss some strange things (without pointing the finger at her).
You can genlty mention her name , for example: we noticed some strange things with samples A and B and with the testsresults from test Y , perhaps we can debate what went wrong? Normally your boss will then ask who did those tests ....
See what I mean..
Dont start accusing her right away yourself because it can backfire + if you cant really catch her..


Also: if she comes in late and leaves early.. its something the boss needs to check, not you.
I know its not honest, but if you start complaining about it...

I   Phusion

(I know you said other suggestions, but figured another vote for Phusion can't be a bad thing )

Trof, on 01 November 2012 - 02:51 AM, said:

I have acces to it. Is there a specific paper you are looking for?

BTW: I find it pretty idiotic that a journal like this is not open acces!

Its not about publishing researchresults, so I do not understand it why this paper is not open acces nor do I understand that it has an impactfactor to be honest.

I recently watched a documentary about a teacher (not this one) that used (rap) songs to help his students. And it turned out that the students got better scores...

Now I found that nice, but on the other hand: if I was a student.. and I had to listen to such songs.. I would feel rather irritated (and it would not stimulate me at all).
It can help some students, but at the same time some students wont like it... I think its important that teachers keep this in mind (for example: do not push students to learn this song if they dont care about it.. everyone has is own style of learning and its important to keep this in mind and not just focus on the weaks for example)

ALso: its nice to use technology, creative presentations etc.. But keep in your mind that this is not the most important thing..
I do not like the evolution I am seeing more and more in schools.
Its true, some things are better and we need to use certain aspects about technology, but dont forget the main issue about schooling.

A big question I always ask: you have 60 minutes to teach something, if you fill in 30 minutes with a movie or song.. you have only 30 minutes left for the rest ... Will you still be able to learn the same amount? Maybe yes, but not sure it is always the case...


A very important thing for me is that a teacher uses a good handbook or hand written text! This has to be very easy to understand and contain many figures/ drawings to understand it.
And of course many excercises with answers.

metionina, on 11 September 2012 - 05:19 AM, said:

Hi,
You want to learn Python? you can find course for leaning it on coursera as Learn to program. you can search for bioinfor part after you learn it.

think about the questions, come up with hypotheses, state them here and we'll give advice.

we won't do all of your thinking for you.

I may run two gels if I am doing both, after loading fresh buffer.  But I do not trust the buffer left over in a gel box others have used.  I even wash the gel box out after I'm done (probably the only time it ever gets washed).

some links you may find useful

http://shop.oreilly....780596000271.do

http://shop.oreilly....780596000806.do


These links have example codes you may try to practice writing or run to see how they look like.

casandra, on 11 September 2012 - 07:50 PM, said:

Casandra, you made my day

Nephrite I understand you totally. I am finishing the first year of my postdoc in a couple of months, and after that I don't know what's gonna happen. I feel lost. I work in a foreign country too, and I can't go back to my own country either because I have been away for nearly 10 years and I don't know anything about the industry or acadmic culture in there. I am not attached to any organization and if I go back I will have to be unemployed for some time until I evaluate my foreign degrees and find a junior lecturer position. It's a shame really.

I think everybody wants to be useful in the society somehow. In our field of science maybe being useful means publish article in high impact factor journals and get a lot of citation. But I didn't have any article until few years ago, so I kept asking myself what if I get hit by a car tomorrow and my life ends with no outcome? I told myself if I don't have articles, then maybe there is another way to be useful...and I found it....I joined Bioforum...first I joined to find my own answers, but later I started answering other people's scientific questions. I started with small questions, questions that I knew the answers for, and left the difficult ones to the moderators or veterans. Towards the end of my PhD I became more and more addicted to this, and I could answer more questions related to the methods and protocols that I use in the lab everyday. I have been here for many years now. If not every day, I have visited this forum every week. I joined when there was no 'like' button , and now I am a moedrator myself. I do this voluntarily. We don't earn anything, but it satisfies me enough. My wife used to get mad at me why I spend too much time here, for something that I don't get salary for, but now she understand my passion and doesn't argue anymore. Who knows, maybe one of the people who I helped becomes the greatest scientist of all time. Maybe he won't remember me, or I won't remember him, but I helped him in his way to success. and that counts.

I don't want to sound philosophical, but I always remember what Isaac Newton said that 'If I can see further, it's because I am standing on the shoulder of giants'. He was right, and everything counts. If there is a God (that I am not sure) he sees everything, and the universe will take record of what we do....That's how I see it.

Disneyland

and for the announcement of the results of the replication of the experiment to prove its repeatability, they'll use baskerville? Perhaps then I'll believe it

and now.....a banged-up version of The Pointers Sisters classic......(triple apologies to them)



Tonight’s the night we’re gonna make it happen
Tonight we put FITC aside
So dearest secondary don’t you worry
Cos when primary binds heck, we’re just fine


[chorus]

I get excited and I just can’t hide it
Ready to produce some photons and I think I like it
I’m so excited and you just can’t quench it
My name’s Alexa fluor and I know we don’t want you… Cy2

You might want to look over the experience of IGEM students using tetR.  Go to the partsregistry page here: http://partsregistry.org/Main_Page
and find the "search parts" link on the right, under "Registry Tools".  Enter "tetR" and find all of the tetR promoters and cassettes listed.  Most of these will be associated with teams.
You can find the team wikis on the igem.org page, and track their success or failure.  You may find parts you'd like already assembled.
If so, you can sign up as a registry lab, and get a (free!) part distribution.

1. you can try to digest your plasmid+insert with an enzyme recognizing a site present only in your insert: only one digestion, only one site in the insert (do you have it?). If your plasmid can linearize you can be sure you have your insert in your plasmid.
2. do a PCR with primers that hybridize on your plasmid just after and before your insert. If you have an amplification you have an insert.
3. use a positive positive control (a PCR of your insert before ligation). Just to be sure that the band you see on the gel is your insert. Use 2% gel and a short time migration. Sometimes primers can be visible on the gel and you see them as a kind of band below 200 pb. Do you use a good marker for small fragments?
4. in order to be sure to have 2 inserts in your plasmid, use primers that hybridize on your plasmid (as point 2 so you can have a 240 pb PCR) or digest your plasmid (2 digestions before and after your insert to linearize the plasmid and show a 240 pb fragment (do not cut in inserts)