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CellBiologist

Member Since 09 Aug 2012
Offline Last Active Jan 28 2013 01:48 PM
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Posts I've Made

In Topic: How to remove mycoplasma contamination in cell lines?

10 August 2012 - 05:43 AM

Hi Curtis,
I am not familiar with which species of mycoplasma are problematic for mammalian cells. Do you think that the multiplex PCR detects mycoplasma species which are not problematic for cell culture?  Any insight is appreciated.
Thanks

In Topic: How to remove mycoplasma contamination in cell lines?

09 August 2012 - 01:55 PM

How we know we have it is somewhat controversial. We have not seen anything by DAPI staining, but when using a PCR kit, some of our stable lines are positive.  Some people in lab think that the PCR kit is too sensitive; others think that if it is positive, there is mycoplasma. I know that ATCC no longer uses PCR to detect mycoplasma, but they sell a kit that does (which is what we used). Have you had any experience with detection of mycoplasma by PCR?
Thanks,
CellBiologist

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