Jump to content

  • Log in with Facebook Log in with Twitter Log In with Google      Sign In   
  • Create Account
  2. → Viewing Profile: Topics: praveen921

praveen921

Member Since 09 Aug 2012
Offline Last Active Mar 08 2013 06:54 AM
-----

Topics I've Started

Cloning at 6 kb gene

07 March 2013 - 05:25 AM

Hello all,
Im trying to Clone a 6KB gene from flower cDNA.Any suggestions before i actually start the work?Im not sure if its gonna be easy amplyfing a 6kn fragment.Is there a good solution to clone this?
Thanks!

TDNA Genotyping PCR program

05 October 2012 - 02:47 AM

Hello all,

I am in search for a PCR program for screening TDNA inserts in plants.The annealing temp of my Primer set is 61C.Could someone help me with the PCR steps i have to use for TDNA genotyping in plants?

Thanks in advance.

Primer Annealing Temperatures

21 August 2012 - 05:54 AM

hello,

I have two different annealing temperatures for my primers (59 and 55). At what annealing temperature should i set when in the PCR when using this primer set ?

Thanks for the help

pUB Vector with two promoters

09 August 2012 - 01:58 AM

Hello all,

I am cloning my gene into a PUB18 vector with a C-terminal RFP fusion. My gene has its own promoter for expression. If i clone the gene along with my promoter into the PUB18 vector, will it still express it as PUB18 has its own promoter as well.I was wondering if the gene expression will be normal in the presence of two promoters in the PUB vector.

Thanks for the help.

  1. BioForum
  2. → Viewing Profile: Topics: praveen921
  3. Privacy Policy
Home - About - Terms of Service - Privacy - Contact Us

©1999-2012 Protocol Online, All rights reserved.