Jump to content

  • Log in with Facebook Log in with Twitter Log In with Google      Sign In   
  • Create Account
  2. → Viewing Profile: Posts: San1986

San1986

Member Since 05 Aug 2012
Offline Last Active Aug 18 2012 10:50 AM
-----

Posts I've Made

In Topic: Conversion of Absorbance into enzyme units

09 August 2012 - 09:15 AM

Thanks bob. I finally explored an option which i didnt know. :)

In Topic: Enzyme storage in eppendorphs to use in enzyme assay next day

09 August 2012 - 08:06 AM

Okay thanks :)

In Topic: Conversion of Absorbance into enzyme units

07 August 2012 - 10:55 AM

I cant find how would i use excel for this? Should i plot both the curves (the standard and the one which is different absorbances of the enzyme) over each other?

In Topic: Enzyme storage in eppendorphs to use in enzyme assay next day

07 August 2012 - 10:53 AM

Okay.
And how can i know if this is a stable enzyme or not? by performing assays?

In Topic: Enzyme storage in eppendorphs to use in enzyme assay next day

06 August 2012 - 11:52 AM

I have read in the literature to store the purified enzyme (post-ammonium sulphate precipitation procedure for proteins) at 4 C because the purified enzyme is unstable. So, if instead of the refrigerator, if i store the crude enzyme at 4 C for the next day use, will the possibilities of native proteases inhibiting the enzyme still be there?

I actually, want to save my time by acquiring crude enzyme by producing it on optimised conditions once for all, for the use in characterization and later on for purification by Ammonium sulphate precipitation method.

San

  1. BioForum
  2. → Viewing Profile: Posts: San1986
  3. Privacy Policy
Home - About - Terms of Service - Privacy - Contact Us

©1999-2012 Protocol Online, All rights reserved.