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biovinz

Member Since 02 Aug 2012
Offline Last Active Apr 14 2013 06:11 AM

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Group Active Members
Active Posts 11
Profile Views 476
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Age Age Unknown
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Gender
Male
Location
Daejeon, South Korea.

About me

My research interests
Gene cloning and Diabetes

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HOYAJM
06 May 2013 - 12:06
pito
14 Apr 2013 - 07:29
Ahrenhase
28 Mar 2013 - 06:35
2xzwei
12 Mar 2013 - 06:11
Akila Wijerathna Yapa
10 Nov 2012 - 11:41

Topics I've Started

Salmonella enteritidis-selective media?

14 April 2013 - 06:11 AM

Hi all,

Can anyone suggest me selective media for isolate salmonella enteritidis from rat blood sample....

Thanks in advance

Vinod

Help for pGEM-T easy vector ligation problem

10 March 2013 - 11:36 PM

Hi all,

I have a trouble with ligation of my insert in pGEM-T easy vector. After transformation, I got blue and white colonies. so i took white colonies and did enzyme digestion but there is no insert..i tried ligation for 1hr at RT, overnight at Rt and overnight at 4C..after that no difference, there is only vector..

Please help me...

Thanks in advance

vinod

Lambda PR promoter sequence

05 March 2013 - 05:52 AM

Dear all,

Plz can any one give me lambda PR promoter sequence

Thanks in advance

Vinod

GST- tagged protein expressed in insoluble

31 December 2012 - 02:06 AM

Dear All,
I am trying to purify a GST-tagged protein. My protein size is around 37 kDa. After IPTG induction, most of the protein expressed in insoluble. how can i get soluble protein? I already tried different temperatures ( 18,25,30 and 37 C) with different induction time(2hr, 3hr and overnight) and different IPTG concentration (0.1, 0.5, 1mM) and different sonication time(10 sec, 20sec, 30 sec, 40 sec 4cycles). I request you to please help me.Looking for your reply.
Thanks in Advance...

VINOD

pLDR20 Vector

28 October 2012 - 06:46 PM

Hi all,
Does anyone have experience with pLDR20 vector. I seen pLDR20 vector map and sequence in some website but i couldn't see terminator. There is no detailed vector map. I am bit confused terminator is there or not? How can i see terminator is there or not in pLDR20 vector. I request you to please help me..
Thanks in advance.

With Kind Regards
VINOD.

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