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Octavina Sukarta

Member Since 02 Aug 2012
Offline Last Active Aug 02 2012 11:36 AM
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Topics I've Started

Alternative to gene silencing for functional assay?? much help needed :)

02 August 2012 - 04:06 AM

Hi,
  I have question regarding functional analysis. I basically have a plant receptor and we'd like to know whether its activity is dependant on a co-receptor in the same plant. To do this our main strategy was to perform transient gene silencing of the co-receptor by siRNA (using hairpin RNA). But unfortunately, the qPCR analysis consistently shows that the co-receptor gene is not being silenced Posted Image ..

Is there an alternative technique that I can use to show that the receptor is dependant on this co-receptor for activity other than silencing?

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